The Gene Komondor has long been a fascinating breed, and recent research has shed new light on its genetics. This breed is known for its unique corded coat.
The Gene Komondor's genetic makeup is a result of centuries of selective breeding. Its distinctive coat is made up of a unique combination of hair types.
Komondors have a relatively low incidence of inherited health issues compared to other breeds.
On a similar theme: Richard Gene
Genetic Analysis Methods
Genetic analysis was used to assess the segregation of the CUBN disease-allele in a family and 3 sporadic cases of Komondors with I-GS.
A convenient DNA genotyping assay was used to produce an amplicon flanking the variant and restriction enzyme (Bce AI) digestion to discriminate the alleles.
The Bce AI digestion was used to indicate the A allele, which was indicated by failure of the PCR amplicon to be digested except at the control Bce AI cut site.
The affected dogs were homozygous A, while the parental dogs were heterozygous A/G, and the clinically normal littermates were homozygous G or heterozygous A/G.
A TaqMan assay was also used to assess Komondor I-GS allele frequencies in North America and Hungary.
The TaqMan assay revealed that 7 of 42 Komondors from North America were heterozygous, and 5 of 56 Komondors from Hungary were heterozygous for the CUBNNM_001003148.1; c.8746 + 1G > A variant.
The allele frequency in North America was 8.3%, and in Hungary it was 4.5%.
Whole Genome Sequencing
Whole Genome Sequencing is a powerful tool used to search for variants in the genome of the Komondor breed. Genomic data were searched for variants using the CanFam 3.1 reference sequence.
The researchers focused on potential candidate genes such as CUBN, AMN, CBLIF, and TCN2. Disruptive variants, homozygous in affected dogs and heterozygous in the parental dog, were prioritized as potential disease-causing candidates.
Functional effects of missense variants were examined by Sorting Intolerant From Tolerant (SIFT) analysis. This analysis helps determine the impact of a missense variant on the protein it codes for.
A specific sequence variant, CUBN c.NM_001003148.1; c.8746 + 1G > A, was genotyped in all members of the I-GS Komondor kindred and sporadic cases.
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